Title : Factor Affecting Enzyme Activity (pH) Objectives 1. To read the following(a) instructions carefully. 2. To determine the significant effects of different pH levels on enzyme activities. 3. Find out the optimal pH execute for the reactions of the given(p) enzyme through a plot graph. Variables Independent variables : pH of source in streak tube Dependent variables : Time for disappearing of colour of the response Constant variables : Total volume of solution in distributively test tube, volume of amylum in apiece test tube, temperature of the solution. Materials Beakers, test tubes in a rack, dropper, grade pen, pipettes, sucker, global indicator paper, stop watch, distilled water, fresh starch solution, iodine solution, amylase, 0,1mol/L hydrochloric astringent (pH=1), 0.1mol/L acetic acerb (pH=3), 0.001mol/L sodium hydrated oxide (pH=11), 0.1 mol/L sodium hydroxide (pH=13), trumpery rod, grooved white tile. Procedures 1. The pH of both battery-acid and two basic was recorded in the table below. 2. 5 test tubes with the solution were set up as shown as in the table below. 3. The contents of each test tubes were sundry(a) and the pH was checked. It was written down to the right of the first in the pH column if the pH is different from the pH recorded. 4.
all the tubes were find ad the time when the blue colour disappeared in each tube was noted. 5. The reciprocal of each time was calculate and the harbor was inserted in the 1/time column. This was used as the exercise of the enzyme. 6. A graph of acti vity (1/time) against pH was plotted on a gr! aph paper. Results Test tubes |mL starch |mL iodine |mL amylase |mL distilled water |mL of acid or basic |pH |Time for fade of colours, t (s) |1/time (s-1) | | | | | | | |Before |After | | | |1 |3 |3 |3 |1 |0 |7 |6 |cl |0.0067 | |2 |3 |3 |3 |0 |1 mL of 0.1 mol/L HCL |1 |3 |240 |0.0042 | |3 |3 |3 |3 |0 |1 mL of 0.1 mol/L acetic acid |3 |5 |195 |0.0051 | |4 |3 |3 |3 |0 |1 mL of 0.001...If you want to get a full essay, put up it on our website: OrderCustomPaper.com
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